anti human junb rabbit monoclonal antibody  (Cell Signaling Technology Inc)

Journal: Developmental cell

Article Title: Sphingosine 1-phosphate receptor signaling establishes AP-1 gradients to allow for retinal endothelial cell specialization

doi: 10.1016/j.devcel.2020.01.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Alexa Fluor 647-conjugated rabbit monoclonal anti-JunB (clone EPR6518) , Abcam , Cat#ab200516;.

Techniques: Purification, Recombinant, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Imaging, Sequencing, RNA Sequencing Assay, Software, Microscopy, Real-time Polymerase Chain Reaction, Injection

Journal: Frontiers in Immunology

Article Title: CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion

doi: 10.3389/fimmu.2018.02989

Figure Lengend Snippet: Partial co-localization of CD103 with endosomal markers in human MoDCs. MoDCs generated in medium alone or in the presence of retinoic acid (RA, 100 nM) were permeabilized and stained with antibodies to CD103 (green) and the following endosomal markers (red): (a–c) clathrin, (e–g) EEA-1, (i–k) Rab11, and (m–o) Rab7a. Images were obtained by confocal microscopy. Co-localization of CD103 with (d) clathrin, (h) EEA-1, (l) Rab11, and (p) Rab7a was calculated as the Manders' colocalization coefficient (M2) using ImageJ. Mean ± SEM of 10 −17 confocal images obtained from two independent experiments. Bar = 20 μm, * P ≤ 0.05; ** P ≤ 0.01; unpaired Student's T -test.

Article Snippet: Endosomal compartments were labeled with rabbit anti-human clathrin (D3C6, Cell Signaling, Danvers, MA), rabbit anti-human EEA-1 (polyclonal), mouse anti-human Rab7a (Rab7-117), and rabbit anti-human Rab11 (polyclonal), all from Abcam, Cambridge, MA.

Techniques: Generated, Staining, Confocal Microscopy